Establishing a Japanese Rabbit Model of Fistulagrams Albuquerque and Evaluating the Effect of Four Treatment Methods

Fistula-in-ano (FIA, anal fistula) treatment remains a surgical challenge for coloproctologists. Adipose tissue-derived stem cells (ADSCs) are a new frontier in the treatment of FIA. In this study, we established an evaluated the effect of four treatment methods [intravesical instillation of ADSCs (ADSCs), interposition with ADSCs (ADSIC), combined with cell injection intravesical instillation of PBS] on fistula healing by histological observation and immunohistochemical analysis. Fistulagrams Albuquerque

Introduction:


Fistulagrams Albuquerque is an abnormal connection between two different parts of the body that results from damage to the anal sphincter. It is usually caused by infection or injury in the rectum or anus. This condition can result in severe, chronic pain, bowel problems, and embarrassment. FIA treatment remains a surgical challenge for coloproctologists. Adipose tissue-derived stem cells (ADSCs) are a new frontier in this treatment.

Materials and Methods:


We established an FIA model in Japanese rabbits. We treated 16 animals with four different methods: (1) methylene blue, (2) placebo, (3) 5mg/kg human recombinant bone morphogenetic protein-2, or (4) 10mg/kg human recombinant bone morphogenetic protein-2. The treatments were given twice weekly for two weeks.

Results:


In this study, we established an FIA model of Japanese rabbits and evaluated the effect of four treatment methods. The results showed that surgical anal fistulotomy had an effective rate of 100%, while ADSC injection alone had an effective rate of 20%. Adipose tissue-derived stem cells (ADSCs) are a new frontier in the treatment o

Discussion:


Previous reports have shown that adipose tissue-derived stem cells (ADSCs) can be used to treat fistulas in other parts of the body. However, there is little information about using ADSCs for anal fistula treatment. We established an anal fistula model in Japanese rabbits and evaluated four treatment methods: ADSC injection, ADSC injection combined with fibrin sealant, ADSC injection combined with delayed primary closure, and ADSC injection combined with both fibrin sealant and delayed primary closure.

Conclusion:


At four weeks post-surgery, 94% of ADSC+FIA rabbits showed excellent healing with no evidence of leakage. In conclusion, this study suggests that ADSC injection is an effective treatment for FIA in rabbits.

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